Figure 4.

CP preconditioning enhances autologous adoptive T cell engraftment after infusion in SHIV-infected NHP animals

Pigtail macaques were first transplanted with autologous HSPCs transduced with Cal-1 vector. Afterward, animals were infected with SHIV-1157ipd3N4 for 6 weeks. Four animals in group 1 were preconditioned with CP, whereas 1 animal in group 2 was not preconditioned before infusion of mock or CAR T cells. To track T cell trafficking, group 1 animals were infused with CellTrace-labeled T cells and the group 2 animal was infused with CellTrace-labeled CAR-T cells 3 days before necropsy. (A) Experimental scheme and time line of NHP adoptive T cell transplant experiment. (B) Lentiviral marking (Cal-1 and CAR) of peripheral blood. (C) CD4zeta CAR gene marking of peripheral blood. (D) Representative FACS plot showing staining for huCD4⁺ CD4CAR T cells in spleen and jejunum of group 1 animal Z14035. (E) Summary of % huCD4⁺ cells among CD45⁺ lymphocytes in lymphoid tissues. (F) Plasma viral load after SHIV infection. Asterisk indicates animals that were sacrificed before completing the study.