FIG. 5.

Inhibition of FMDV infectivity by peptides A15 and A15 with RGG instead of RGD. The amino acid sequences of peptides A15 and A15-RGG are given in Materials and Methods. BHK-21 cell monolayers (about 5 × 10⁵ cells) were treated with the indicated concentration of peptide A15 or A15-RGG for 45 min at room temperature and further incubated in the presence of FMDV (50 to 150 PFU) for 45 min at 37°C. The monolayers were washed with 0.1 M phosphate buffer (pH 6.0) and overlaid with agar containing medium as described in Materials and Methods. The FMDVs used in different experiments are listed in the right; hs- denotes a clone selected for its inability to bind heparin. Origins of the viruses and procedures for selection of clones and infectivity assays are described in Materials and Methods. Each value is the average of three determinations. Standard deviations are indicated.