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tsProt.A procapsids before (a) and after (b) incubation at 21°C for 72 h. Procapsids were isolated by antibody precipitation as described in Materials and Methods from BHK cells infected for 15 h at 39°C (the NPT). Note that before incubation (a) procapsids are round in profile, indicating they have not angularized, while after incubation (b) capsid profiles show angles indicating they have the mature, icosahedral structure. Bar = 1,500 Å.
