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. 2011 Oct 24;103(1):50–57. doi: 10.1111/j.1349-7006.2011.02112.x

Figure 3.

Figure 3

 Knockdown of RUVBL1/2 and Tel2 interacting proteins partially affects the phosphatidylinositol 3‐kinase‐related protein kinase (PIKK) abundance. (A,C,D) HeLa TetOff cells were transfected with the indicated siRNA. Sixty hours after transfection, total cell lysates were analyzed by western blotting with the indicated antibodies. Same knockdown samples were used in panels A, C, D in Figure 3, and panels A and B in Figure 4. Mean values of the relative protein levels compared to the nonsilencing (NS) control from two independent experiments are shown under the blots. (B) HeLa TetOff cells were treated with vehicle or 2 μM 17‐AAG for 12 h and total cell lysates were analyzed by western blotting with the indicated antibodies. To estimate the protein abundance, 33 and 11% of the NS sample (A,C,D) or untreated sample (B) were loaded. ATM, ataxia telangiectasia mutated; ATR, ATM‐and Rad3‐related; DNA‐PKcs, DNA‐dependent protein kinase catalytic subunit; mTOR, mammalian target of rapamycin; SMG‐1, suppressor with morphological effect on genitalia 1; TRRAP, transformation/transcription domain‐associated protein.