Table 2.

Polymerase chain reaction (PCR) results for cat infected with Anaplasma platys (Apl), ‘Candidatus Mycoplasma haemominutum’ (‘CMh’), Bartonella henselae San Antonio 2 (BhSA2) and Bartonella koehlerae (Bk)

Sample	Collection date	PCR results
		Ehrlichia/Anaplasma	Apl	Apl	Mycoplasma	Bartonella	Bk species-specific
		(16S rRNA)	(p44)	(GroEL)	(16S rRNA)	(16–23S ITS)	(16–23S ITS)
Wb	22 December 2011	Apl	Apl	Apl	‘CMh’	Neg	Neg
Wb	1 April 2012	Apl	Apl	Apl	‘CMh’	Neg	Bk *
Lc	1 April 2012	Neg	Neg	Neg	NA	NA	NA
Wb	6 April 2012	Neg	Neg	Neg	‘CMh’	Neg	Neg
Lc	6 April 2012	Neg	Neg	Neg	‘CMh’	Neg	Bk*
Wb	25 May 2012	Neg	Neg	Neg	‘CMh’	BhSA2*	Neg
Wb	1 July 2012	Neg	Neg	Neg	‘CMh’	BhSA2 †	Neg
Wb	1 January 2013	Neg	Neg	Neg	‘CMh’	Neg	Neg
Wb	1 April 2013	Neg	Neg	Neg	‘CMh’	Neg	Neg

All amplicons were sequenced to confirm genus and species identity

ITS = intergenic transcribed spacer; Wb = whole blood; Lc = leukocyte-rich fractions; Neg = negative; NA = not available

^*Bartonella amplicon from blood

^†Bartonella amplicon from Bartonella alpha-proteobacteria growth medium enrichment blood culture