Figure 6.
Overexpression of BMI‐1 stabilizes survivin expression post‐translationally. (a) BMI‐1, survivin and β‐actin mRNA expression in BMI‐1‐transduced and mock‐transduced HT and RL cells determined by RT‐PCR. (b) The proteasomal inhibitor MG132 stabilized the expression of survivin in mock‐transduced cells. RL‐mock cells (1 × 105) were plated and treated with 20 μM of MG132 for 16 h. Densitometric analyses on ratio of survivin expression/β‐actin are shown in the upper panel. Western blotting pictures on survivin expression are shown in the lower panel.