Figure 2.

 Effects of the forced overexpression of (a,b) Pim‐3 and (c,d) Pim‐1 on cell numbers of MiaPaca‐2 (a,c) and PCI66 (b,d) cells in the presence of T26. Cells were transfected with Pim‐3 or Pim‐1 cDNA or control vectors as described in the Materials and Methods. After transfection, cells were incubated in the presence of 15 μmol/L T26 for the times indicated. Cell numbers were determined and cell lysates were subjected to immunoblotting with anti‐Pim‐3, anti‐Pim‐3, or anti‐β‐actin antibodies, as indicated. Relative cell numbers were determined compared with Day 0 and data are shown as the mean ± SD. Experiments were repeated three times. *P < 0.01.