Figure 4.

 Lymphocytes genetically engineered to express MAGE‐A4‐specific T‐cell receptor‐maintained specific reactivity after in vivo passage. Non‐obese, diabetic/SCID/γc^null mice (n = 4 per group) were subcutaneously inoculated with 2.5 × 10⁶ KE4 tumor cells, then intravenously administered 1 × 10⁸ (A) or 5 × 10⁷ (B) gene‐modified (■) or unmodified (○) cells on day 0. Mononuclear cells were purified from peripheral blood collected from mice on the indicated days. Intracellular γ‐interferon (IFN‐γ) production by these cells was assessed after being stimulated with 1 μM MAGE‐A4_141–153 peptide for 6 h. Data are shown as the percentage of IFN‐γ‐producing cells within the total human CD8⁺ cell population. Results are representative of three independent experiments.