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Aerosol inhalation is an effective method to increase NP and siRNA localization to the lungs. a) Representative ex vivo pictures of the lung with orthotopic tumor. b) ex vivo images show that Cy5.5-siRNA NPs or free Cy5.5-siRNA could be specifically accumulated in lung at 24 h after administration through aerosol inhalation (1 mg/kg Cy5.5-siRNA, 400 μL).
