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a: Design of the 3-HKT inverted repeat construct used for Chlamydomonas chloroplast transformation. The two black arrows indicate the binding sites of primers used for PCR confirmation of Chlamydomonas chloroplast transformants. b: PCR confirmation of Chlamydomonas chloroplast transformants. -ve: water control, C: non transgenic parent strain (CC-4147), 1-15: Chlamydomonas chloroplast transformants, +ve: plasmid control as template.
