Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 27;4:6. doi: 10.5281/zenodo.10894766

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Kumar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Figure 2. — Effect of feeding Chlamydomonas chloroplast trans-formants expressing 3-HKT dsRNA on Anopheles stephensi larvae. a) An.stephensi larval mortality observed with Chlamydomonas chloroplast (CC-4147/pCVAC108) transformants expressing 3-HKT dsRNAs. Twenty (1^st experiment) or 30 (2^nd experiment) newly hatched An. stephensi larvae were reared on algae plus 1/3^rd yeast+ sera micron (1/3^rd amount of yeast+micron mixture fed to control larvae). Control larvae were reared on non-transgenic algae with a yeast+sera micron mixture diet. Data shown are the average of two experiments. For clone 108-13 results were identical (40% mortality) in both the experiments, hence there is no variation. b) Different phenotypes exhibited by An. stephensi larvae following feeding on transgenic algae producing 3-HKT dsRNA. 1) Dead larvae of An. stephensi reared on transgenic algae. 2) Comparison of a larva exhibiting growth inhibition (red arrow) upon feeding on transgenic algae to a normally developing larva (green arrow) on day 12. 3) Image of a dead pupa (red arrow) and a live pupa (green arrow) in an experimental well with transgenic algae.