FIG. 7.

Infectivity of mutant EnvAs. EnvA-MLV pseudotyped virions encoding β-galactosidase were prepared as for Fig. 4. Serial dilutions of pseudotyped viral supernatants were added to Tva-expressing NIH 3T3(PG950) cells, incubated at 37°C for 48 h, and assayed for β-galactosidase as described in Materials and Methods. Data from at least three independent experiments were averaged and are presented as a function of increasing hydrophobicity of the mutant residue. Ranking of relative hydrophobicity is described in the footnote to Table 2. Error bars represent standard error of the mean.