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. 2024 Jun 10;12:RP91876. doi: 10.7554/eLife.91876

Figure 2. Airway smooth muscle was substantially reduced in Bmpr1a conditional knockout (CKO) lungs.

Figure 2.

(A) Scatter plots of RNA-seq analysis for differentially expressed genes (DEGs) (n=3). (B) RPKM heatmap of Muscle System Process Genes (GO:0003012). Genes involved in the Muscle System Process with significant changes between Bmpr1a CKO and wildtype (WT) lungs are shown in the RNA-seq heatmap. The input data was RPKM values generated from the raw data using the R/Bioconductor program ‘edgeR’. (C and D) Whole mount immunostaining of Cdh1 and Acta2 in embryonic day (E)14.5 and E15.5 lungs. Excessive dilation of airway terminals and extensive reduction of airway smooth muscle were observed in E15.5 Bmpr1a CKO lungs. The normal-looking airways are marked by arrows and the enlarged epithelial bud accompanied with compromised smooth muscle are marked by arrowheads. (E) E15.5 lung section stained with Cdh1& Acta2 or Myh11. *Vascular structures.