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. 2024 Jun 10;12:RP91876. doi: 10.7554/eLife.91876

Figure 3. Bmpr1a conditional knockout (CKO) resulted in a significant reduction in elastin expression underneath the airway epithelia of embryonic day (E)15.5 lungs, but not in the pericytes, vasculature, and basement membrane.

Figure 3.

(A) Expression of Cspg4, Pecam1, Lama1, Lama2, Col3a1, and Eln at the mRNA level was measured by real-time PCR, *p<0.05. (B) E15.5 lung section stained with Cspg4, Pecam1, laminin, and Col3a1. (C) E15.5 lung section stained with Cdh1, Acta2, and elastin. (D) Reduced elastin expression of Bmpr1a CKO lungs at the protein level was detected by western blot (WB). (E and F) Elastin expression in E15.5 lung mesenchymal cells was upregulated by BMP4 and downregulated by BMP type 1 receptor-specific inhibitor LDN193189 (LDN), as detected by WB and immunofluorescence staining.

Figure 3—source data 1. Original file for the western blot (WB) analysis in Figure 3D (anti-Eln and anti-GAPDH).
Figure 3—source data 2. PDF containing Figure 3D and original scans of the relevant western blot (WB) analysis (anti-Eln and anti-GAPDH) with highlighted bands and sample labels.
Figure 3—source data 3. Original file for the western blot (WB) analysis in Figure 3E (anti-Eln).
Figure 3—source data 4. Original file for the western blot (WB) analysis in Figure 3E (anti-GAPDH).
Figure 3—source data 5. PDF containing Figure 3E and original scans of the relevant western blot (WB) analysis (anti-Eln and anti-GAPDH) with highlighted bands and sample labels.