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. 2024 Jun 10;12:RP91876. doi: 10.7554/eLife.91876

Figure 5. Bmpr1a-mediated signaling played different roles in the differentiation of airway versus vascular smooth muscle cells (SMCs).

Figure 5.

(A) YFP and DsRed expression patterns in the fetal lungs of Tagln-YFP/Cspg4-DsRed mice. a: airway, v: vessel. (B) Airway SMCs (YFP+) and vascular SMCs (YFP+/DsRed+) were isolated by fluorescence-activated cell sorting (FACS). (C) The differential effect of Bmp4 treatment (50 ng/ml) on contractile protein Myh11 expression was analyzed in SMCs of non-vascular origin (YFP+) versus vascular origin (YFP+/DsRed+), and the role of Bmpr1a in mediating this effect was tested by adding its specific inhibitor LDN193189 (200 nM). The western blot (WB) data of Myh11 expression was normalized to GAPDH (loading control) and is represented as a relative change to the control condition, *p<0.05.

Figure 5—source data 1. Original file for the western blot (WB) analysis in Figure 5C (anti-Myh11).
Figure 5—source data 2. Original file for the western blot (WB) analysis in Figure 5C (anti-GAPDH).
Figure 5—source data 3. PDF containing Figure 5C and original scans of the relevant western blot (WB) analysis (anti-Myh11 and anti-GAPDH) with highlighted bands and sample labels.