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. 2024 Jun 10;15:4772. doi: 10.1038/s41467-024-48663-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a mRNA levels of WRN normalized by GAPDH mRNA (n = 4). b Representative flow cytometric histogram of γ-H2AX staining in healthy-, WS-, and gcWS-iMφs without oxLDL treatment (left) and γ-H2AX MFI (right) (n = 4). c Immunofluorescence image of γ-H2AX foci in healthy-, WS-, and gcWS-iMφs. The scale bar is 30 μm. d Absolute numbers of CD14⁺CD11b⁺ healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3). e Representative flow cytometric plots of annexin V staining in healthy-, WS-, and gcWS-iMφs without (left top) or with (left bottom) oxLDL treatment. Bar graphs show the total proportion of annexin V⁺ cells among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 3), before (right top) and after (right bottom) oxLDL treatment. f mRNA levels of CDKN1A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 4). g Representative flow cytometric plots of SA-β-gal staining before (left) and after (right) oxLDL treatment among healthy-, WS-, and gcWS-iMφs. Bar graphs show the MFI of SA-β-gal among healthy- (n = 3), WS- (n = 4), and gcWS-iMφs (n = 4), before (left) and after (right) oxLDL treatment. h mRNA levels of CDKN2A normalized by GAPDH mRNA before (left) and after (right) oxLDL treatment (n = 3). i Secreted pro-inflammatory cytokine protein levels, determined by ELISA, for healthy-, WS-, and gcWS-iMφs before (n = 9, 10, 4) and after oxLDL treatment (n = 9, 7, 4). Three independent experiments of three independent biological samples were used for Healthy- ans WS- iMφs. Data are shown as the mean ± standard error of the mean (SEM) of biologically independent samples unless otherwise stated. One-way ANOVA with Tukey’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein. Source data are provided as a Source Data file.