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. 2024 Jun 10;15:4772. doi: 10.1038/s41467-024-48663-w

Fig. 7. DHX58-dependent dsRNA sensing pathway initiates transcription of type I IFN signature genes.

Fig. 7

a Schematic diagram of DHX58-dependent dsRNA sensing pathway. b Representative FACS plot of dsRNA accumulation (left) and MFI of dsRNA accumulation (right) in healthy- (n = 6), WS- (n = 6), and gcWS-iMφs (n = 4), three biologically independent samples over two independent experiments. c Representative FACS plot of MAVS expression (left) and MFI of MAVS expression (right) in healthy-, WS-, and gcWS-iMφs (n = 4). d Percent knockdown efficiency of lentiviral transduction of shDHX58 in healthy-iMφs (n = 3) (left) and WS-iMφs (n = 4) (right). mRNA levels of type I IFN signature genes normalized by GAPDH mRNA after lentiviral transduction with shDHX58 in WS- iMφs (n = 5) (e) and healthy-iMφs (n = 3) (f). Data are shown as the mean ± SEM biologically independent. One-way ANOVA with Dunnett’s multiple comparisons was performed to calculate the p values. MFI mean fluorescent intensity, oxLDL oxidized low-density lipoprotein, MAVS mitochondrial antiviral-signaling protein, IFN interferon. Source data are provided as a Source Data file.