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. 2024 Apr 8;31(6):697–710. doi: 10.1038/s41418-024-01288-4

Fig. 1. PRTN3-deficiency induces myeloid differentiation in mice.

Fig. 1

a–d messenger RNA (mRNA), Protein level detected by WB and ELISA, and immunofluorescence staining of Prtn3 were determined in neutrophils and LSK cells from Prtn3−/− and WT mice (n = 3); e Representative flow cytometry plots for neutrophils, monocytes, T cells and B cells in bone marrow from WT and Prtn3−/− mice. Numbers denote the frequency of each population among live singlets(n = 7). f Representative flow cytometry plots for neutrophils, monocytes, T cells, and B cells in bone marrow from the CD45.2 cells in CD45.1 recipient mice (n = 7). Numbers denote the frequency of each population among live singlets. g, h Quantification of the frequency of CD45.1, CD45.2, the ratio of CD45.2/CD45.1, neutrophils, monocytes, T cells, and B cells in the CD45.2 recipient mice (n = 7). scale bars, 50 μm; *p < 0.05, **p < 0.01, ***p < 0.001. Data are the mean ± s.d.; n: biologically independent experiments. Statistical analysis was performed using an unpaired two-tailed Student’s t test.