Expression of viral envelope proteins. Proteins from microglia, total CNS, bone marrow, peritoneal macrophages, and spleen from rats and 3T3 cells, respectively, were subjected to immunoblotting and probed with a virus-specific anti-envelope antibody. In microglia, higher amounts of the uncleaved precursor protein, Pr85, than of the cleaved outer membrane portion, gp70, of the viral envelope protein were found. In control cells (3T3) and in total rat brain as well as in all other rat organs, mainly gp70 was found.