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. 2024 Mar 21;11(22):2310159. doi: 10.1002/advs.202310159

Figure 4.

Figure 4

Assays of the interactions between Ma1α and Ma1β in plant cells. A) Bimolecular fluorescence complementation (BiFC) assays of interactions among and between Ma1α and Ma1β in N. benthamiana leaves infiltrated with A. tumefaciens harboring Ma1α‐nYFP/Ma1α‐cYFP, Ma1β‐nYFP/Ma1β‐cYFP, Ma1α‐nYFP/Ma1β‐cYFP or Ma1β‐nYFP/Ma1α‐cYFP fusion constructs, with a previously characterized, tonoplast‐localized protein ALS3[ 29 ] as a negative control. Vacuoles were released after lysis of N. benthamiana protoplasts transiently transformed with the constructs indicated. Bars = 15 µm. B) Luciferase (LUC) complementation imaging assays of the interactions of Ma1α and Ma1β in N. benthamiana leaves infiltrated with the constructs indicated. Ma1α and Ma1β were fused in frame with nLUC and/or cLUC. Three independent biological replicates were performed. C) Co‐immunoprecipitation (IP) assays of the interactions among and between Ma1α and Ma1β in N. benthamiana leaves co‐transformed with the expression constructs indicated using MYC and HA antibodies.