Figure 7.

a) Treatment scheme of an orthotopic CT26 colorectal tumor model using mGP70‐loaded LNPs. (b and c) Photographs and weights of isolated tumors (n = 5 biological independent samples). Statistical significance was calculated by unpaired two‐tailed Student's t‐test. d) Kaplan–Meier survival curves of mice after different treatments (n = 11–14 biologically independent samples). The percentages of CD3⁺CD8⁺ and CD3⁺CD4⁺ T cells in mLN e and f) and tumor g and h) of treated mice. The population of CD3⁻NK1.1⁺ i), CD3⁺NK1.1⁺ j), and CD86⁺F4/80⁺ cells among tumor cells. k) M1/M2 ratio in the tumor microenvironment (n = 5 biological independent samples for all flow cytometry analysis). Data are shown as mean ± SD. Statistical analysis was calculated using one‐way ANOVA and Tukey's post‐hoc test. ^* p < 0.05, ^** p <0.01, ^*** p <0.001, ^**** p <0.0001, n.s. represents not statistically significant.