Table 2. Instrument Parameters.

MS survey scan
spray source	positive mode
spray voltage	2.650 kV
ion transfer tube temperature	250 °C
MS1 orbitrap resolution	120,000
MS2 orbitrap resolution	30,000
full MS scan cycle	350–1650 m/z
RF lens (%)	40
AGC (%)	300
maximum injection time mode	custom
maximum injection time	20 ms
source fragmentation	disabled

MS survey scan followed by MS/MS DIA Scan events
multiplex ions	false
collision energy mode	stepped
collision energy type	normalized
HCD collision energies (%)	25.5,27,30
orbitrap resolution	30,000
first mass	200
RF lens (%)	40
AGC target	custom
normalized AGC target (%)	3000
maximum injection time mode	custom
maximum injection time	55 ms