Fig. 1.

Synthesis of sGFP using T. themophilus S30 extracts and different types of DNA template. A Reaction mixtures containing 40 ng/µl of pET28b_sGFP (circles) or the same molar concentration of sGFP PCR amplicon (squares) were incubated at 50 °C for 70 min. As a negative control of the reaction, an empty pET28b vector was used (triangles). Composition of the reaction mixtures are indicated in Table 1. sGFP synthesized was monitored in real time as fluorescence emission. B Different amounts of template DNA (in ng/ml) were tested as in (A), with the corresponding amounts of empty vector as negative controls. Results are the average of n = 3 reactions and error bars represent standard deviations