Fig. 4.

Coupling cell-free protein synthesis with an enzymatic activity. Reaction mixtures containing 40 ng/µl of pET22b_TTP0042 and 5 µM of either FDGlu (triangles) or FDC (inverted triangles) were first incubated at 50 °C for 2 h and then, temperature was increased to 70 °C for 17 h. An empty pET22b plasmid was incubated in the presence of FDGlu (circles) or FDC (squares) as negative controls of the reactions. Composition of the reaction mixtures are indicated in Table 1