Table 1.

Composition of the reaction mix for thermostable IVTT

		Final concentration
Buffer Mix	HEPES–KOH (pH = 8)	50 mM
	Magnesium glutamate (MgGlut)	10 mM
	Potassium glutamate (KGlut)	100 mM
	Dithiothreitol (DTT)	1.5 mM
	Amino acids (aa mix)	1.5 mM
	Phosphoenolpyruvate (PEP)	15 mM
	Pyruvate kinase (PK)	0.22 μM
	Nucleoside diphosphate kinase (NDK)	0.070 μM
	Inorganic pyrophosphatase (PPA)	0.040 μM
	Adenylate kinase (ADK)	0.26 μM
	Folinic acid	0.068 mM
	Murine RNase inhibitor	1 U/μl
	Total E. coli tRNAs	0.2 mg/ml
	ATP/GTP	2 mM
	UTP/CTP	1.2 mM
	Spermine	2 mM
Template DNA		40 ng/μl
Thermostable T7 RNA polymerase		1 U/μl
T. thermophilus S30 extract		4.7 mg/ml