FIG. 2.

Confocal photomicrographs of the kinetics of appearance and distribution of capsid (VP5), glycoprotein (gC), and tegument (VP16) antigens in human neurons at selected times p.i. (A) VP5 at 2 h p.i., showing retrograde transport within an axon to the cell body. (B) VP5 at 6 h p.i., showing no detectable antigen. Photographic sensitivity was enhanced on this image to allow visualization of the cell. Under the photographic conditions used for preparation of the rest of this panel, no cell staining would normally be seen. (C) VP5 at 13 h p.i. showing intense nuclear staining and antigen diffusely distributed in the cytoplasm, extending into the axon hillock. (D) VP5 at 17 h p.i. showing cytoplasmic and axonal distribution. The arrow indicates the principal axon. Bar, 25 μm. (E) gC at 13 h p.i. showing cytoplasmic and axonal distribution. Distal regions of the stained axon (arrow) are out of the confocal plane. (F) gC at 17 h p.i. showing disappearance of antigen from the Golgi and cytoplasm. Bar, 10 μm. (G) VP16 at 13 h p.i. showing that this protein was present only in the cytoplasm. (H and J) VP16 at 15 h p.i. showing distribution in only the proximal axon. Note the antigen front (arrow). Under bright-field microscopy, the axon continued distally (for 120 μm) beyond this front (arrow) (J). Bar in panel J, 10 μm. (I) VP16 at 17 h p.i. showing that this protein is present in both the cytoplasm and the full length of the axon.