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Assembly of JCV recombinant particles in the nucleus of COS-7 cells transfected with VP231-SRα. A large number of round particles (40 nm in diameter) and filamentous forms (30 nm in diameter) of the recombinant capsids were detected in the nucleus. The morphological features of these particles were identical to those of JCV Tokyo-1 strain in oligodendrocytes of the brain of a Japanese PML patient (43, 44). Magnification: top, ×10,000; center, ×20,000; bottom, ×40,000.
