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. 2024 Jun 10;65(6):16. doi: 10.1167/iovs.65.6.16

Table.

Sequence of Primers for RT-qPCR

Gene Species Direction Primer Sequence 5′→3′
GAPDH Human Forward TGCCCTCAACGACCACTTTG
Reverse CTGGTGGTCCAGGGGTCTTA
IL-1β Human Forward TCATACCAAGGAGAAGTAATAAGCC
Reverse ACCAAAGAAGTACAGCGCCAT
β-actin Mouse Forward GATTACTGCTCTGGCTCCTAGC
Reverse GACTCATCGTACTCCTGCTTGC
IL-1β Mouse Forward GCAACTGTTCCTGAACTCAACT
Reverse ATCTTTTGGGGTCCGTCAACT
COL3A1 Mouse Forward AAGGCTGCAAGATGGATGCT
Reverse GTGCTTACGTGGGACAGTCA
α-SMA Mouse Forward CAGCGGGCATCCACGAAAC
Reverse CCTGACAGGACGTTGTTAGCAT
FN1 Mouse Forward ACCATCTACGTCATTGCCCTG
Reverse ACCAGTTGGGGAAGCTCATC
Vim Mouse Forward TTCTCTGGCACGTCTTGACC
Reverse GCTTGGAAACGTCCACATCG
MiR-29b-3p Mouse Forward CTGCCGTAGCACCATTTGA
Mouse Reverse TATCCTTGTTCACGACTCCTTCAC
Human Forward CTGCCGTAGCACCATTTGA
Human Reverse TATCCTTGTTCACGACTCCTTCAC
U6 Forward GCTTCGGCAGCACATATACTAAAAT
Reverse CGCTTCAGAATTTGCGTGTCAT