Table 1. Advantages and disadvantages of microscopy techniques for the study of molecule dynamics.

Microscopy techniques	Advantages	Disadvantages
Whole cell 4D fluorescence reconstruction	Cheap;	Bulk visualization;
	Whole-cell visualization;	Need of structured illumination microscopy or image deconvolution.
	Chance to follow receptor internalization.
Fluorescence Recovery After Photobleaching (FRAP)	Fast identification of mobile and immobile molecular fractions;	Characterization of a restricted area of the cell;
	Easy definition of Diffusion Coefficient.	Photodamage;
		Need of Confocal Laser Scanning Microscopy (CLSM).
Single Particle Tracking (SPT)	Visualize the position of single molecule and record its trajectories;	Need of Total Internal Reflection Fluorescence (TIRF) microscopy;
	High temporal and spatial resolution	Large amount of data to analyze.