Fig 7. Silencing suppression activity of cp-rt, cp, cp-rt-ugg and cp-rt-ugg-mutzf in an agroinfiltration assay.

(A) gfp-c3 was introduced by agroinfiltration into N. benthamiana leaves along with a plasmid encoding a candidate VSR (right half of leaf) or the empty plasmid (EP) (left half of leaf). (B) Agroinfiltrated N. benthamiana leaves were imaged under UV light at 4 dpi. The agroinfiltrated constructs are indicated above the images. (C) RT-qPCR analysis of gfp mRNA accumulation. Nb ACT-b (GI:380505031) was used as a reference gene. Values represent the mean +/− SD (n = 4). (D) Northern blot analyses depicting the accumulation of gfp-specific siRNAs. A hydrolysed Cy5 labelled in vitro transcribed RNA fragment complementary to gfp was used as a probe. 5S ribosomal RNA was used as a loading control. (E–G) As for panels A–C except mgfp5 and dsGF were used instead of gfp-c3.