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. 2024 May 30;20(5):e1012034. doi: 10.1371/journal.ppat.1012034

Fig 8. The different course of infection of AV2, AV2-2st and AV2-mutZF in wild type and RDR6i N. benthamiana plants.

Fig 8

(A) Detection of CP by western blot in wild type N. benthamiana (left panels) and RDR6i N. benthamiana (right panels) infected with AV2, AV2-2st or AV2-mutZF. Samples were collected at 7 dpi from the 2nd upper non-inoculated leaf, and at 14 and 21 dpi from the 3rd upper non-inoculated leaf. Sizes of molecular weight markers are indicated on the right. Ponceau red staining of the large Rubisco subunit (RbcL) was used as a loading control. (B) RT-qPCR analysis of RNA3 accumulation at the same time points. Nb ACT-b (GI:380505031) was used as a reference gene. Values are expressed in arbitrary units and represent the mean +/− SD (n = 5).