Fig. 1.

Mutated ras-induced PLD1 expression.

PLD1, PLD2 and β-actin proteins of HT-29, Caco-2, DLD-1, and HCT 116 cells are shown. Results of ras activation assay performed according to Materials and Methods are also illustrated. (b) PLD1 and PLD2 mRNA levels were measured by the semi-quantitative RT-PCR as described in Materials and Methods. Cycle # denotes the number of PCR cycles. Relative PLD1 and PLD2 mRNA levels are described as the ratio of PLD/GAPDH. Relative mRNA levels of Caco-2 are regarded as 1.0, respectively. Results of PLD protein and PLD mRNA levels of original NIH3T3, mock-NIH3T3 and mutated Hras-NIH3T3 cells are also illustrated. Anti-pan Ras antibody was used to confirm the overexpression of mutated Hras. In the case of NIH3T3, the internal control of semi-quantitative RT-PCR was β-actin. Relative mRNA levels of mock-NIH3T3 are regarded as 1.0.