TABLE 1.

Extracellular virus production from neurons versus that from nonneuronal cells^a

Type of cell	d.p.i.	% Infected cellsb	PFU/mlc	PFU/1,000 infected cellsd
Primary CD46+ mouse neuron	1	5	NDe	0
	2	5	20	10
	3	10	20	6
Vero	1	80	ND	0
	2	100	6.0 × 104	600
	3	100	3.2 × 105	1,600
HeLa	1	50	360	10
	2	80	7.2 × 103	130
	3	100	1.6 × 104	170
NT2	1	50	54	2
	2	85	36	1
	3	90	156	3
Undifferentiated NT2	1	60	40	1
	2	75	6.4 × 103	130
	3	95	9.2 × 103	100

^aCells plated at a density of 1 × 10⁵ to 2 × 10⁵ cells/coverslip were infected 1 day later with MV Edmonston (MOI = 1). Data represent results from at least two separate experiments, each performed in duplicate. 

^bPercent infected cells was determined by immunohistochemical staining of MV antigen. 

^cSupernatants tested for infectious virus by plaque assay. 

^dPFU/1,000 cell values, used to correct for cell division rates in fibroblast cultures (cell counts determined in parallel uninfected cultures) and the proportion of cells infected at the indicated time, were calculated as {[(PFU/ml) × 4 ml]/(number of cells × proportion infected)} × 1,000. 

^eND, not detected.