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. 2024 Jun 11;15:4778. doi: 10.1038/s41467-024-48916-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Distribution of UMIs per nucleus in the entire snRNA-seq dataset. nUMI/nuclei: number of UMIs detected in each nuclei. B Distribution of peaks per nucleus in the entire snATAC-seq dataset. nPeaks/nuclei: number of peaks detected per nuclei in the multiomics dataset. C Correlation between flight (y-axis) and ground control (x-axis) single nuclei multiomics samples (Pearson’s correlation coefficient, r = 0.95; p < 0.05) shown as a scatter plot. This is a two-sided Pearson correlation test with 95% confidence intervals performed on the average expression (log(1 + avgUMI)). avgUMI: average UMI counts per spot. D UMAP of single nuclei multiomics data and cluster annotations. E 11 functional multiomics clusters categories represented by their marker genes. F Distribution of UMIs per spot for the whole spatial transcriptomics (ST) dataset. nUMI/spot: number of UMIs detected per spot in the ST dataset. G Distribution of unique genes per spot for the whole spatial transcriptomics (ST) dataset. nGenes/spot: number of genes detected per spot in the ST dataset. H Correlation between flight (y-axis) and ground control (x-axis) ST samples (Pearson’s correlation coefficient, r = 0.99; p < 0.05) shown as a scatter plot. This is a two-sided Pearson correlation test with 95% confidence intervals performed on the average expression (log(1 + avgUMI)). avgUMI: average UMI counts per spot.