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. 2024 Jun 11;10:278. doi: 10.1038/s41420-024-02040-0

Fig. 3. PDD traps prexasertib-induced PARylated proteins on chromatin.

Fig. 3

A Immunoblot analysis showing PARylation of proteins, pH2AX(S139), pCHK1(S296), and CHK1 basally as well as after treatment with 5 nM prexasertib, 5 µM PDD and their combination in OC cells. B Immunoblotting showing pRPA32(S33) in SKOV3 cells treated with DMSO, 5 nM prexasertib, 5 µM PDD and their combination. C Immunofluorescence study showing PAR foci staining in SKOV3 cells treated with DMSO, 5 nM prexasertib, 5 µM PDD and their combination. D Histogram representation of PAR foci staining in SKOV3 cells treated with DMSO, 5 nM prexasertib, 5 µM PDD and their combination. 50 cells were counted for each group for an experiment, and the data presented are an average of 3 different experiments. All the experiments were repeated three times, and the bar graph denotes their standard deviation. One-way ANOVA using Dunnett’s T3 multiple comparison tests were performed to analyze the statistical significance. ***p < 0.001; ****p < 0.0001.