FIG. 5.

Binding of full CPV particles to uninduced and induced Mv1-K44A cells. (A) Purified CPV full particles were bound to uninduced or induced Mv1-K44A cells for 1 h at 4°C; then the cells were fixed, and capsids were detected by indirect immunofluorescence with MAb 8. Phase-contrast images are shown in the left panels. The two fluorescence images were collected using identical exposures. (B) Mv1-K44A cells grown in the presence or absence of tetracycline were suspended by treatment with EDTA and then incubated for 1 h on ice with or without 40 μg of full CPV particles per ml. After washing, the cells were fixed and immunostained for capsid and then permeabilized, and the dynamin K44A was immunostained using the fused HA epitope tag. Cell-associated fluorescence was determined by flow cytometry for each sample. The histograms shows the binding of CPV to the cell surface. The inset is a dot plot showing the binding of CPV versus expression of the HA tag.