TABLE 2.
Experimental design using CCD on the gallic acid yield of mango seed kernel.
| Run order | X 1 pH | X 2 Solvent ratio (mL/g) | X 3 Extraction temperature (°C) | X 4 Extraction time (mins) | Y a Gallic acid (mg/g) |
|---|---|---|---|---|---|
| 1 | 3 (−1) | 20 (−1) | 50 (+1) | 50 (+1) | 2.82 ± 0.08 |
| 2 | 4 (0) | 30 (0) | 40 (0) | 20 (−2) | 4.99 ± 0.03 |
| 3 | 5 (+1) | 40 (+1) | 30 (−1) | 50 (+1) | 3.62 ± 0.05 |
| 4 | 4 (0) | 30 (0) | 60 (+2) | 40 (0) | 4.97 ± 0.04 |
| 5 | 4 (0) | 30 (0) | 40 (0) | 40 (0) | 5.29 ± 0.05 |
| 6 | 5 (+1) | 40 (+1) | 50 (+1) | 30 (−1) | 4.63 ± 0.05 |
| 7 | 4 (0) | 50 (+2) | 40 (0) | 40 (0) | 2.73 ± 0.05 |
| 8 | 3 (−1) | 20 (−1) | 30 (−1) | 50 (+1) | 2.25 ± 0.09 |
| 9 | 4 (0) | 30 (0) | 40 (0) | 40 (0) | 5.45 ± 0.06 |
| 10 | 6 (+2) | 30 (0) | 40 (0) | 40 (0) | 4.43 ± 0.05 |
| 11 | 3 (−1) | 40 (+1) | 30 (−1) | 30 (−1) | 4.71 ± 0.08 |
| 12 | 5 (+1) | 40 (+1) | 50 (+1) | 50 (+1) | 3.12 ± 0.04 |
| 13 | 4 (0) | 10 (−2) | 40 (0) | 40 (0) | 0.43 ± 0.04 |
| 14 | 5 (+1) | 20 (−1) | 30 (−1) | 50 (+1) | 3.19 ± 0.02 |
| 15 | 5 (+1) | 20 (−1) | 30 (−1) | 30 (−1) | 3.25 ± 0.11 |
| 16 | 3 (−1) | 40 (+1) | 50 (+1) | 50 (+1) | 4.56 ± 0.29 |
| 17 | 3 (−1) | 40 (+1) | 30 (−1) | 50 (+1) | 2.89 ± 0.05 |
| 18 | 5 (+1) | 20 (−1) | 50 (+1) | 30 (−1) | 3.07 ± 0.02 |
| 19 | 4 (0) | 30 (0) | 20 (−2) | 40 (0) | 4.25 ± 0.06 |
| 20 | 5 (+1) | 40 (+1) | 30 (−1) | 30 (−1) | 5.66 ± 0.10 |
| 21 | 2 (−2) | 30 (0) | 40 (0) | 40 (0) | 3.44 ± 0.07 |
| 22 | 4 (0) | 30 (0) | 40 (0) | 40 (0) | 5.11 ± 0.02 |
| 23 | 3 (−1) | 20 (−1) | 50 (+1) | 30 (−1) | 2.57 ± 0.09 |
| 24 | 5 (+1) | 20 (−1) | 50 (+1) | 50 (+1) | 3.85 ± 0.12 |
| 25 | 3 (−1) | 20 (−1) | 30 (−1) | 30 (−1) | 1.89 ± 0.04 |
| 26 | 4 (0) | 30 (0) | 40 (0) | 60 (+2) | 5.10 ± 0.05 |
| 27 | 3 (−1) | 40 (+1) | 50 (+1) | 30 (−1) | 5.82 ± 0.03 |
a
Gallic acid yield is expressed as the mean ± SD.