FIG. 3.
DexS inhibition of gp120-CXCR4 interactions. (A) A3.01 (CD4+ CXCR4+) T cells were preincubated with various concentrations of DexS prior to the addition of HIV-1MN gp120. The CXCR4-specific MAb 12G5 or the CD4-specific MAb Q4120 was subsequently added. (B) A3.01 cells were preincubated with DexS prior to addition of MAb 12G5 or Q4120. Bound MAb was detected by indirect immunofluorescence and flow cytometry, and the results are expressed as percent inhibition of MAb binding relative to the positive (no inhibitor) and negative (no MAb) controls. Each datum point is the mean of values obtained from triplicate samples.