Fig. 5.

The ratio of pT73-Rab10 to total Rab10 in serum is elevated in mouse models of late-onset PD. (A) Levels of total LRRK2, (B) total Rab10 and (C) the ratio of pT73-Rab10 to total Rab10 as measured by SiMOA in venous serum from VPS35^WT/WT (2 males and 4 females) mice, VPS35^WT/D620N (4 males and 4 females) mice, and VPS35^D620N/D620N (6 males) mice. (D) Graphs show numbers of neutrophils (CD11b + Ly6G+), (E) classical monocytes (CD11b + Ly6G- Ly6C+), and (F) CD4 T-cells measured by flow cytometry in VPS35^WT/WT and VPS35^D620N/D620N serum samples. (G) Representative immunoblots and quantification of cultured bone marrow derived macrophages demonstrating elevated pT73-Rab10 to total Rab10 levels with mutant VPS35 expression. (H) Levels of total LRRK2, (I) total Rab10 and (J) the ratio of pT73-Rab10 to total Rab10 from Snca^−/− (3 males and 6 females) mice, and PAC-SNCA/Snca^−/− (5 males and 4 females) mice. (K) Graphs show numbers of neutrophils (CD11b + Ly6G+), (L) classical monocytes (CD11b + Ly6G- Ly6C+), and (M) CD4 T-cells measured by flow cytometry in Snca^−/− and PAC-SNCA/Snca^−/− mice blood. (N) Representative immunoblots and quantification of cultured bone marrow-derived macrophages demonstrating low but equivalent pT73-Rab10 to total Rab10 between Snca^−/− and PAC-SNCA/Snca^−/− mice. Mouse ages ranged from 3–6 months. Columns depict group mean, dots represent the mean values from an animal or independent experiment, and error bars show SEM. P values are from two-tailed t-tests or one-way ANOVA analysis with Tukey’s multiple comparison when three groups are tested