Table 2.
Plasmids used in this study
| Plasmids | Description | Source |
|---|---|---|
| pACYCDuet | Two T7 promoters, p15A replicon, CmR, low copy number, 10 ~ 12 copies/cell | Novagen |
| pETDuet | Two T7 promoters, pBR322 replicon, AmpR, medium copy number, ~40 copies/cell | Novagen |
| pRSFDuet | Two T7 promoters, RSF1030 replicon, KanR, high copy number, ~100 copies/cell | Novagen |
| FGF21/pACYCDuet | pACYCDuet containing FGF21 gene | This study |
| FGF21/pETDuet | pETDuet containing FGF21 gene | This study |
| FGF21/pRSFDuet | pRSFDuet containing FGF21 gene | This study |
| rhClp/pACYCDuet | pACYCDuet containing rhClp gene, highly repetitive gene | This study |
| rhClp/pETDuet | pETDuet containing rhClp gene, highly repetitive gene | This study |
| rhClp/pRSFDuet | pRSFDuet containing rhClp gene, highly repetitive gene | This study |
| T7-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-P | This study |
| T7-T7-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-T7-P | This study |
| T7-P-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-P-P | This study |
| T7-T7-P-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-T7-P-P | This study |
| T7-P-T7-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-P-T7-P | This study |
| T7-P-P-T7-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-P-P-T7-P | This study |
| T7-P-P-T7-P-P | pETDuet containing FGF21 gene, The plasmid construction mode was T7-P-P-T7-P-P | This study |