Fig. 4.

(a) Confocal images of a $30\times 30\mu \mathrm{m}$ FOV centered on the same location where DyMIN1-DyMIN4 and conventional STED 50-image sequences (see Table 1 for imaging parameters) were taken of a 45 nm fluorescent bead sample with $10\times 10\mu \mathrm{m}$ field and $512\times 512\text{pixels}$, after the DyMIN/STED sequences were acquired, as labeled. (b) Confocal image versus DyMIN1 image with a select bead lineout shown in panel (c) indicating a smaller FWHM of the bead with DyMIN super resolution imaging. Similar results are found for DyMIN2-4 (not shown). (d) Confocal image versus conventional STED image with a selected bead lineout shown in panel (e) with similar reduction in the FWHM. Colorbars are scaled to the maximum value of the image. Scalebars are $2\mu \mathrm{m}$.