Figure 3.

Expression of AIMs in blood-derived CD4/CD8α-defined T cell subsets after stimulation with ASFV Estonia 2014. (A) Representative FCM plots depicting expression of CD25, CD69, CD40L, ICOS and TNF-α in CD4⁻CD8α⁺, CD4⁺CD8α⁺ and CD4⁺CD8α⁻ T cells. PBMC samples were incubated with mock inoculum (Mock, left columns) or ASFV Estonia 2014 (ASFV, right columns) for 18 h. Cells were pre-gated in the following order: live, single and CD3⁺. Gates shown indicate gating applied to calculate frequencies of AIM⁺ and TNF-α⁺ cells. Dot size was enlarged to improve visibility of AIM⁺ and TNF-α⁺ cells. (B) Frequencies of CD25⁺, CD69⁺, CD40L⁺, ICOS⁺ and TNF-α⁺ cells within CD4/CD8α-defined T cell subsets in mock-inoculated (Mock) and ASFV Estonia 2014 (ASFV)-stimulated samples. Each dot represents data from one animal (n = 5). Asterisks indicate significant differences between groups (^*p ≤ 0.05, ^**p ≤ 0.01).