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. 2024 May 29;11:1390486. doi: 10.3389/fvets.2024.1390486

Figure 4.

Figure 4

Relative distribution of AIM phenotypes within CD4/CD8α-defined T cell subsets in SEB-stimulated vs. ASFV-stimulated PBMC cultures. (A) Doughnut charts of AIM phenotypes in SEB-stimulated samples generated by Boolean gating in CD4−CD8α+ (top row), CD4+CD8α+ (middle row) and CD4+CD8α− (bottom row) T cells. Each doughnut represents the PBMC sample of one pig. Different phenotypes are indicated by different colors with all AIM phenotypes co-expressing TNF-α summarized in gray. CD25−CD40L−CD69−TNF-α− and CD25 single+ T cell phenotypes are not shown. (B) Doughnut charts of AIM phenotypes in ASFV Estonia 2014-stimulated samples generated by Boolean gating in CD4−CD8α+ (top row), CD4+CD8α+ (middle row) and CD4+CD8α− (bottom row) T cells. Each doughnut represents the PBMC sample of one pig. Different phenotypes are indicated by different colors with all AIM phenotypes co-expressing TNF-α summarized in gray. CD25−CD40L−CD69−TNF-α− and CD25 single+ T cell phenotypes are not shown.