Extended Data Fig. 7. Schematic of AAV spread and fiber placements in Agrp-IRES-Cre::NPY-KO, Agrp-IRES-Cre::vGat^lox/lox and Agrp-IRES-Cre::NPY-KO::vGat^lox/lox mice.

a, Schematics representing AAV spread (shaded regions) and fiber placements (rectangles) for every animal related to experiments in Fig. 4a-c. Each animal is represented by a different colour. b-d. Representative images of ChR2-mCherry expression in AgRP neuron somas and fiber placement in the arcuate nucleus. Scale bar = 200 μm. 3V = Third ventricle. The schematics were created using The Mouse Brain in Stereotaxic Coordinates Second Edition (Paxinos and Franklin).