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. 2024 May 7;13(1):2352434. doi: 10.1080/22221751.2024.2352434

Figure 4.

Figure 4.

Detection of binding antibodies targeting MPXV. Indirect multiple ELISAs were conducted using purified recombinant MPXV A35 (A) and H3 (B) antigens were used to detect MPXV-specific binding antibodies in pig sera. Serum was diluted in 3x serial dilutions. An ELISA titer was considered positive when the OD450 was greater than 3 standard deviations above the mean of naïve animal sera run concurrently. (C) Indirect ELISA using crude whole VACV. Plates were coated with either VACV or BSC-40 cell lysate. Sera was diluted 1:100, 1:200, and 1:400. An ELISA titer was considered positive when the average OD450 among two replicates was greater than 2 standard deviations above the mean of corresponding samples in the cell lysate plate.

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