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Co-localization of ASC-GFP and NLRP3. Co-localization was assessed after triggering NLRP3 inflammasome formation with either 5 mM ATP (2h), 100µg/mL MSU (5h) or 10µM nigericin (30min) in PMA-differentiated, LPS-primed THP-1-ASC-GFP cells. Cells were imaged using the EVOS™ M7000 Imaging System, 40X objective and DAPI and GFP light cube. Scale bars are 1µm. Created with BioRender.com.
