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. 2024 May 29;10(11):e32023. doi: 10.1016/j.heliyon.2024.e32023

Fig. 1.

Fig. 1

ASC-speck dynamics differ between triggers. ASC-speck count in PMA-differentiated, LPS-primed THP-1 cells after triggering with ATP, MSU or nigericin. ASC-GFP specks were imaged every 30 min for 21 h by fluorescence microscopy and automatically quantified using the Weka segmentation plugin for ImageJ. Inset shows ATP triggered ASC-speck counts during the first 11 h. Data are represented as mean (solid line) ± SEM (shaded area), n = 5. Created with BioRender.com.