FIG. 1.

Effect of IFN pretreatment on accumulation of viral mRNAs. Vero cells were mock treated (−) or pretreated (+) with 1,000 U of IFN-α per ml for 16 h prior to infection with 5 PFU of the indicated virus per cell. Total RNA was harvested either 4, 8, or 24 h postinfection by using Trizol (Gibco BRL). Aliquots (5 μg) were then analyzed for ICP4, ICP27, or ICP8 transcript levels by Northern blot analysis with ³²P random-primer-labeled probe by using ExpressHyb (Clontech).