TABLE 1.
Oligonucleotides used for the PCR amplification of complete or partial RTBV ORFs
| ORF | Oligonucleotidesa |
|---|---|
| P1 |
AAGTCTTACCCGGGT GTTCCAAAGAGGGATCTT-86 (+) |
| _______ | |
| AACATGGATCCGCGCTCATGTAGCTTGATG-654 (−) | |
| _______ | |
| P2 | TAAGCATCAAGCCATATGATGCGCTGATTAC-679 (+) |
| _______ | |
| TAGAAGTACCAGCATATGGTCTAAGGCTCA-995 (−) | |
| _______ | |
| MP | GTAAACCCGGGCAAACATATGAGCCTTAGACCATTT-1011 (+) |
GACA TTATTCTATAGATTTGGC-2371 (−) |
|
| _______ | |
| 40-CP | CGCGGATCCAGCCATGGCCAAATCTATAGAAGAAAT-2390 (+) |
| _______ | |
| GGCGAATTCCAAGATCTTATATTTCAAGGAAATTTTCAA-3443 (−) | |
| _______ | |
| PR | ACGCGAATTCACAACCTCATTAGCTATGAGAGCAAAT-3849 (+) |
| _______ | |
| ACGCGGATCCTTAATATCCTAATATTGGTTGAGGCTG-4384 (−) | |
| _______ | |
| RT-RH p55 | ACCGGAATTCAAGAATGAAATAGGAAATCAAAGT-4431 (+) |
| _______ | |
| ACGCGGATCCTTATTTTAAAATAAAATCTCCCTCACG-5827 (−) | |
| RT-RH p62 |
ACCG AAGAATGAAATAGGAAATCAAAGT-4431 (+) |
ACGC TTAGGCACTCTTTTCCTTTAGCCTTTC-5995 (−) |
|
| P4 |
GATC ATCCATATGAATATAGAGTACCCG-6059 (+) |
GCTTT ACCCGGGTTAAGCATTGTCCATACG-7194 (−) |
a
The sequence of the oligonucleotides is given 5′ to 3′. RTBV-derived sequences are underlined, and the position of the 3′-end nucleotide of each oligonucleotide in the plus- or minus-strand RTBV sequence is indicated on the right. The restriction sites used for cloning are overlined. In some cases, additional restriction sites are present but not indicated.