TABLE 2.
Agroinoculations of rice with RTBV gene II mutants
| Plasmida | P2 versionb | No. of plants infected/no. inoculatedc in:
|
Infection (%)d | |
|---|---|---|---|---|
| Expt 1 | Expt 2 | |||
| pRTRB1162 | P2 | 11/15 | 20/25 | 77 |
| pRT-P2M5 | P2M5 (Δ108–110) | 8/15 | 21/25 | 69 |
| pRT-P2M8 | P2M8 (R106A) | 10/15 | 19/25 | 72 |
| pRT-P2M11 | P2M11 (I104A) | 0/15 | 0/25 | 0 |
| pRT-P2M13 | P2M13 (K101A; K102A) | 9/15 | 19/25 | 68 |
a
Plasmid constructs used to transform A. tumefaciens.
b
P2 variants encoded by the RTBV mutants. Modifications are indicated in parentheses.
c
Infection was detected by testing the plants for the presence of the RTBV gene II product and the viral genome.
d
The values correspond to the mean percentages of infected plants in the two independent experiments.